Potential Inhibitory Effects of Rhizopus oligosporus on the Growth of Aspergillus flavus FNCC6109 in Corn Seeds

Corn (Zea mays L.) has the highest carbohydrate content after rice but is contaminated easily by Aspergillus flavus with its Aflatoxin, which decreases corn quality. Chemical control of A. flavus has side effects, so there is a need for safe control techniques, namely biological control. This study aimed to determine the potential of the culture filtrate of Rhizopus oligosporus in the control of A. flavus FNCC6109 on corn kernels carried out in vitro and in vivo. In vitro testing was carried out using the dual culture method after R. oligosporus incubated for 3, 4 and 5 days. While the in vivo treatment was carried out by giving culture filtrate to corn kernels with a concentration of 0, 10, 20, 30, 40 and 50% (v/v) into corn kernels. In vitro tests were carried out by measuring the diameter of the colonies of A. flavus FNCC6109 on PDA media that give culture filtrate, while in vivo testing was determined by the plating method. The results showed that the culture filtrate of R. oligosporus significantly (P ≤ 0.05) inhibited A. flavus FNCC6109 both in vitro and in vivo. This study concluded that R. oligosporus could inhibit the growth of A. flavus with the highest inhibition percentage at 67.27 ± 2.70 with a 5-day incubation period, and the lowest was at 61.27 ± 5.13 with a 3-day incubation period.