UJI EFEKTIVITAS EKSTRAK BUAH APEL MANALAGI (Malus sylvestris Mill) KONSENTRASI 25%, 50%, DAN 100% TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans SECARA IN VITRO

Dental caries is spread throughout the world and can cause disorders in the body, such as impaired mastication, food absorption, and digestion. Of the several types of bacteria, Streptococcus mutans is the species most frequently found and is the main cause of dental caries. One natural resource that can be used as an alternative ingredient to reduce the number of Streptococcus mutans bacteria includes Manalagi Apple (Malus sylvestris Mill). The purpose of this research is to determine the effectiveness of Manalagi Apple (Malus sylvestris Mill) fruit extract in inhibiting the growth of Streptococcus mutans bacteria. This research is laboratory research with a Post Test Control Group Design. The samples used were Streptococcus mutans bacteria which were divided into 5 groups, namely the group treated with Manalagi Apple (Malus sylvestris Mill) fruit extract with concentrations of 25%, 50% and 100%, the group treated with ChKM as a positive control, and the group treated with aquadest as negative control. The resulting inhibitory power at 25% concentration was 10.2 mm, at 50% concentration was 13.12 mm, and at 100% concentration was 15.47 mm. The higher the concentration of the extract, the greater the amount of antibacterial compounds released. It can be concluded that the inhibitory power of Manalagi Apple (Malus sylvestris Mill) fruit extract at concentrations of 25%, 50% and 100% can inhibit the growth of Streptococcus mutans bacteria, with the most effective concentration being in the treatment group containing Manalagi Apple fruit extract at a concentration of 100%.