UJI EFEKTIVITAS DAYA HAMBAT EKSTRAK DAUN SIRSAK (ANNONA MURICATA L.) TERHADAP PERTUMBUHAN CANDIDA ALBICANS

Abstrak

Rongga mulut merupakan pintu awal masuknya makanan dan minumansebagai sumber nutrisi dan menjadi tempat yang rentan serta sering terjadiperadangan ataupun infeksi yang disebabkan oleh mikroorganisme. Salah satuinfeksi yang cukup banyak dijumpai di rongga mulut yaitu infeksi jamur sepertiCandida albicans. Oral candidiasis merupakan salah satu infeksi oleh jamurpatogen opportunistik yaitu Candida albicans. Chlorhexidine gluconate 0,2%merupakan obat kumur antiseptik yang dapat digunakan untuk pengobatan oralcandidiasis. Chlorhexidine gluconate 0,2% memiliki beberapa efek samping dalampenggunaannya. Daun sirsak memiliki aktivitas farmakologis yaitu antijamurberdasarkan kandungan senyawa aktifnya yang dapat dijadikan alternatif obatherbal. Penelitian ini bertujuan untuk mengevaluasi efektivitas antijamur ekstrakdaun sirsak (Annona muricata L.) terhadap pertumbuhan jamur Candida albicansdengan konsentrasi 25%, 50%, 75%, dan 100%. Metode penelitian yang digunakanadalah metode difusi untuk menentukan aktivitas ekstrak daun sirsak (Annonamuricata L.) terhadap jamur. Hasil penelitian didapatkan rerata zona daya hambatkontrol positif sebesar 23,53 mm, konsentrasi 50% sebesar 11,45 mm, konsentrasi75% sebesar 14,28, dan konsentrasi 100% sebesar 18,13 mm. Berdasarkan hasilpenelitian, ekstrak daun sirsak (Annona muricata L.) dengan konsentrasi 50%, 75%,dan 100% mampu menghambat pertumbuhan jamur Candida albicans.

Abstract

The oral cavity is the initial gateway for food and beverage to enter as asource of nutrition and is a place that is vulnerable and often causes inflammationor infection caused by microorganisms. One infection that is quite often found inthe oral cavity is fungal infections such as Candida albicans. Oral candidiasis isone of the infections by opportunistic pathogenic fungi, namely Candida albicans.Chlorhexidine gluconate 0.2% is an antiseptic mouthwash that can be used for theoral treatment of candidiasis. Chlorhexidine gluconate 0.2% has several side effectsin its use. Soursop leaf have pharmacological activity, namely antifungal based ontheir active compound content which can be used as an alternative herbal medicine.This study aims to evaluate the antifungal effectiveness of soursop leaf extract(Annona muricata L.) against the growth of the Candida albicans fungus atconcentrations of 25%, 50%, 75% and 100%. The research method used was thediffusion method to determine the activity of soursop leaf extract (Annona muricataL.) against fungi. The research results showed that the average zone of positivecontrol inhibition was 23.53 mm, the 50% concentration was 11.45 mm, the 75%concentration was 14.28, and the 100% concentration was 18.13 mm. Based onresearch results, soursop leaf extract (Annona muricata L.) with concentrations of50%, 75% and 100% is able to inhibit the growth of the Candida albicans fungus