Biological Mediator Addition Increased Dentinosialo-Phospoprotein and Ameloblastin Expression of Human Dental Pulp Stem Cells

Dental pulp stem cells are one of mesenchymal stem cell that are very important in the field of regenerative medicine. It is necessary to identify the expression level of specific genes in human dental pulp stem cells (hDPSCs). Dentinosialo-phosphoprotein (DSPP) is an odontogenic marker gene in odontoblastic differentiation, which plays a role in the process of tooth organogenesis. Ameloblastin (AMBN) is a specific gene protein found in tooth enamel and important in the process of differentiating epithelial cells of teeth into ameloblasts to form enamel. One biological mediator in the field of dentistry is enamel matrix derivative (EMD). This study determines the effect of EMD on the DSPP and AMBN expression level of hDPSCs.
This study used hDPSCs from canines and premolars, mRNA expression of DSPP and AMBN was measured by Real-Time PCR after being treated with EMD and without EMD.
DSPP and AMBN showed the highest expression level in EMD addition within 24 hours, and there was a significant difference (p<0.05) between the treatment group and the control group.
The present study showed that biological mediator significantly increased DSPP and AMBN expression of hDPSCs