Effectiveness of piper betel leaf extract against the growth of Staphylococcus aureus (in vitro)

Infection of the oral cavity is an infection caused by the activity of pathological microorganisms such as Staphylococcus aureusbacteria. The use of herbal ingredients such as betel leaf extract, in the assumption, can be useful for inhibiting the growth ofStaphylococcus aureus in the infection. This study aims to determine the effectiveness of antibacterial piper betel leaf extract 25%against the growth of Staphylococcus aureus. This research used posttest only control group design, composed of 27 samples ofStaphylococcus aureus type A bacteria which were divided into 3 treatment groups, consists of : group I treated with 25% betel leafextract, group II treated with lemongrass extract 25% and control group treated with Amoxicillin 500 mg. Result obtained by meansparametric statistic test, that is one way-ANOVA, obtained p value 0.001 (p<0.05), so there is a significant difference in the inhibitionzone of the three groups. Result of Post Hoc Test, that is Least Significant Difference (LSD) obtained p value 0.001 (p<0.05), thereis significant difference in the measurement of inhibition zone between groups. Piper betel leaf extract is 25% more effective thanlemongrass extract 25% and amoxicillin 500 mg against the growth of Staphylococcus aureus.